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LL37 and shock waves

LL37 and shock waves


 

 

 

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LL37 and Shock Waves

 

 

 

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antimicrobial peptides

Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.

 

 

 

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antimicrobial peptides

Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.

LL37 is an antimicrobial peptide which is released upon 

mechanical stress. It builds complexes with RNA and can thus

activate Toll-like receptors and mediate inflammation.

 

 

 

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hypothesis

LL37 is released upon SWT and complexes extracellular.

 

 

 

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RNA levels are increased after SWT

finding no. 1

RNA Nanodrop no vortex 141114

R

N

A

 (n

g

/m

l)

CT

R

SW

1h

SW

2h

SW

5h

SW

48

h

SW

72

h

0

50

100

150

 

 

 

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finding no. 2

TLR3 activation

CT

R

SW

T

SW

T+

RN

as

e

0

50000

100000

150000

***

***

RNAse is not able to remove RNA in the supernatant of

treated cells

 

 

 

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finding no. 3

combined treatment with proteinase and RNAse is able to remove RNA from supernatant and abolishes SW effect

TLR3 activation

CTR

SWT

SWT+RNase

SWT+Prot.K+RNase

0

50000

100000

150000

***

***

-> RNA has to be complexed by a protein (like LL37)

 

 

 

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finding no. 4

Protein analysis reveals significantly higher levels of LL37 in SW treated cells !!!

CTR

SWT 30′     SWT 1h      SWT 2h SWT 5h    SWT 24h  SWT 48

Click here to read more.