LL37 and shock waves
LL37 and Shock Waves
antimicrobial peptides
Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.
antimicrobial peptides
Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.
LL37 is an antimicrobial peptide which is released upon
mechanical stress. It builds complexes with RNA and can thus
activate Toll-like receptors and mediate inflammation.
hypothesis
LL37 is released upon SWT and complexes extracellular.
RNA levels are increased after SWT
finding no. 1
RNA Nanodrop no vortex 141114
R
N
A
(n
g
/m
l)
CT
R
SW
T
1h
SW
T
2h
SW
T
5h
SW
T
48
h
SW
T
72
h
0
50
100
150
finding no. 2
TLR3 activation
CT
R
SW
T
SW
T+
RN
as
e
0
50000
100000
150000
***
***
RNAse is not able to remove RNA in the supernatant of
treated cells
finding no. 3
combined treatment with proteinase and RNAse is able to remove RNA from supernatant and abolishes SW effect
TLR3 activation
CTR
SWT
SWT+RNase
SWT+Prot.K+RNase
0
50000
100000
150000
***
***
-> RNA has to be complexed by a protein (like LL37)
finding no. 4
Protein analysis reveals significantly higher levels of LL37 in SW treated cells !!!
CTR
SWT 30′ SWT 1h SWT 2h SWT 5h SWT 24h SWT 48