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Home » Research » LL37 and shock waves

LL37 and shock waves

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LL37 and Shock Waves

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antimicrobial peptides

Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.

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antimicrobial peptides

Lai Y, Gallo RL. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol.
2009;30(3):131-41.

LL37 is an antimicrobial peptide which is released upon

mechanical stress. It builds complexes with RNA and can thus

activate Toll-like receptors and mediate inflammation.

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hypothesis

LL37 is released upon SWT and complexes extracellular.

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RNA levels are increased after SWT

finding no. 1

RNA Nanodrop no vortex 141114

R

N

A

(n

g

/m

l)

CT

R

SW

T

1h

SW

T

2h

SW

T

5h

SW

T

48

h

SW

T

72

h

0

50

100

150

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finding no. 2

TLR3 activation

CT

R

SW

T

SW

T+

RN

as

e

0

50000

100000

150000

***

***

RNAse is not able to remove RNA in the supernatant of

treated cells

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finding no. 3

combined treatment with proteinase and RNAse is able to remove RNA from supernatant and abolishes SW effect

TLR3 activation

CTR

SWT

SWT+RNase

SWT+Prot.K+RNase

0

50000

100000

150000

***

***

-> RNA has to be complexed by a protein (like LL37)

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finding no. 4

Protein analysis reveals significantly higher levels of LL37 in SW treated cells !!!

CTR

SWT 30′ SWT 1h SWT 2h SWT 5h SWT 24h SWT 48

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